1.
Determination of ellagic acid in fresh and processed fruits by HPLC
Amakura, Y.; Okada, M.; Tsuji, S.;Tonogai, Y.
JOURNAL- FOOD HYGIENIC SOCIETY OF
Abstract : No abstract is available for this item.
2.
Identification of ellagic acid conjugates and other polyphenolics in
Joon-Hee Lee, Jodie V Johnson, Stephen T Talcott
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 53 (15) 2005: 6003-6010
Abstract:
Ellagic acid, ellagic acid glycosides, and ellagitannins found in various fruits and nuts, including muscadine grape, are reported to have potential health-promoting benefits and antioxidant properties. This study isolated and identified several ellagic acid derivatives present in muscadine grapes and determined their relative antioxidant properties (AOX). Compounds were extracted from grape skins and pulp using methanol, and the solvent was evaporated. Isolates were dissolved in citric acid buffer (pH 3.5) and absorbed onto C18 cartridges. Nonretained polyphenolics were collected separately and again partitioned from Sephadex LH-20, whereas retained polyphenolics were first eluted with ethyl acetate followed by methanol. Ellagic acid derivatives were identified on the basis of UV and mass spectra, and the presence of ellagitannins was confirmed by a significant increase in free ellagic acid with HPLC followed by acid hydrolysis. Muscadine grapes contained phenolic acids, flavonols, anthocyanins, ellagic acid, and numerous ellagic acid derivatives. AOX varied in the order ethyl acetate > methanol > C18 nonretained fractions; each correlated to both total phenolics (r = 0.90) and total ellagic acid (r = 0.99) contents. Results of this study revealed previously unidentified ellagic acid derivatives in muscadine grapes.
Simultaneous analysis of ellagic acid and coenzyme Q”1”0 by derivative spectroscopy and HPLC
Ratnam, D Venkat; Bhardway, V; Kumar, M N V Ravi
Talanta , 70(2) 2006: 387-391
Abstract: No abstract is available for this item.
Determination of ellagic acid in oak leaves and in sheep ruminal fluid by ion-pairRP-HPLC
JOURNAL OF CHROMATOGRAPHY B ,855 (2) 2007 : 276-279
Abstract:
An isocratic ion-pair high-performance liquid chromatography (IP-RP-HPLC) method with UV detection was developed to identify and quantify ellagic acid (EA). This phenolic compound is widely distributed in the plants and is often present in the diet of ruminants. The method was validated and validation parameters were: linearity range 5-100mg/L; correlation coefficient, 0.9995; mean recoveries (99.94 and 101.07%) and detection limit 1.4mg/L. Method was applied for the determination of ellagic acid in oak leaves and in ruminal fluid from to a vitro ruminal system. The proposed method proved to be rapid and accurate and can be successfully used in ruminant nutrition studies.
5.
Identification of ellagic acid conjugates and other polyphenolics in muscadine grapes by HPLC-ESI-MS.
Lee JH, Johnson JV, talcott ST.
Journal of agricultural and food chemistry, 53 (15)2005: 6003-6010
Abstract:
Ellagic acid, ellagic acid glycosides, and ellagitannins found in various fruits and nuts, including muscadine grape, are reported to have potential health-promoting benefits and antioxidant properties. This study isolated and identified several ellagic acid derivatives present in muscadine grapes and determined their relative antioxidant properties (AOX). Compounds were extracted from grape skins and pulp using methanol, and the solvent was evaporated. Isolates were dissolved in citric acid buffer (pH 3.5) and absorbed onto C18 cartridges. Nonretained polyphenolics were collected separately and again partitioned from Sephadex LH-20, whereas retained polyphenolics were first eluted with ethyl acetate followed by methanol. Ellagic acid derivatives were identified on the basis of UV and mass spectra, and the presence of ellagitannins was confirmed by a significant increase in free ellagic acid with HPLC followed by acid hydrolysis. Muscadine grapes contained phenolic acids, flavonols, anthocyanins, ellagic acid, and numerous ellagic acid derivatives. AOX varied in the order ethyl acetate > methanol > C18 nonretained fractions; each correlated to both total phenolics (r = 0.90) and total ellagic acid (r = 0.99) contents. Results of this study revealed previously unidentified ellagic acid derivatives in muscadine grapes.
Simultaneous analysis of ellagic acid and coenzyme Q10 by derivative spectroscopy and HPLC
Ratnam, D.V. / Bhardwaj, V. / Kumar, M.N.V.R.
Talanta, 70 (2)Sept 2006:387-391
Abstract:
Antioxidants are gaining tremendous interest as chemopreventive as well as chemotherapeutic agents. Ellagic acid (EA) is a plant derived compound with very poor solubility in water and very low octanol/water partition coefficient and coenzyme Q10 (CoQ10) is a highly lipophilic compound, which is synthesized in the body and can be derived from food supplements as well. The new insights in the combination therapy are promising a better future in many challenging diseases. Synergism is among the key advantages of combination therapy apart from decreased intensity of unwanted effects of a compound, increased patient compliance and reduction in cost of therapy. EA and CoQ10 supplementation in combination will be beneficial in strengthening the weakened antioxidant defense system in many diseases related to oxidative stress. Here we report first derivative UV spectroscopic and HPLC methods for the simultaneous analysis of these two agents in pharmaceutical preparations. Results obtained indicate that the derivative spectroscopy is as efficient as HPLC method in quantitative analysis. Retention of ellagic acid can be increased using PEG bonded column which is poorly retained on C18 column. PEG column can be used for rapid simultaneous analysis of EA and CoQ10, which are having diverse physicochemical properties.
Keywords: Ellagic acid; Coenzyme Q10; Derivative spectroscopy; HPLC; PEG bonded column
7.
Determination of ellagic acic in oak leaves and in sheep ruminal fluid by ion-pair RP-HPLC
del Moral, P García / Arín, M J / Resines, J A / Díez, M T
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 855 (2), p.276-279, Aug 2007.
Abstract:
An isocratic ion-pair high-performance liquid chromatography (IP-RP-HPLC) method with UV detection was developed to identify and quantify ellagic acid (EA). This phenolic compound is widely distributed in the plants and is often present in the diet of ruminants. The method was validated and validation parameters were: linearity range 5-100 mg/L; correlation coefficient, 0.9995; mean recoveries (99.94 and 101.07%) and detection limit 1.4 mg/L. Method was applied for the determination of ellagic acid in oak leaves and in ruminal fluid from to a vitro ruminal system. The proposed method proved to be rapid and accurate and can be successfully used in ruminant nutrition studies.
Simultaneous analysis of ellagic acid and coenzyme Q(10) by derivative spectroscopy and HPLC.
Ratnam, D Venkat / Bhardwaj, V / Kumar, M N V
Talanta, 70 (2) Sep 2006:387-391
Abstract:
Antioxidants are gaining tremendous interest as chemopreventive as well as chemotherapeutic agents. Ellagic acid (EA) is a plant derived compound with very poor solubility in water and very low octanol/water partition coefficient and coenzyme Q(10) (CoQ(10)) is a highly lipophilic compound, which is synthesized in the body and can be derived from food supplements as well. The new insights in the combination therapy are promising a better future in many challenging diseases. Synergism is among the key advantages of combination therapy apart from decreased intensity of unwanted effects of a compound, increased patient compliance and reduction in cost of therapy. EA and CoQ(10) supplementation in combination will be beneficial in strengthening the weakened antioxidant defense system in many diseases related to oxidative stress. Here we report first derivative UV spectroscopic and HPLC methods for the simultaneous analysis of these two agents in pharmaceutical preparations. Results obtained indicate that the derivative spectroscopy is as efficient as HPLC method in quantitative analysis. Retention of ellagic acid can be increased using PEG bonded column which is poorly retained on C(18) column. PEG column can be used for rapid simultaneous analysis of EA and CoQ(10), which are having diverse physicochemical properties.
9.
Determination of ellagic acid in oak leaves and in sheep ruminal fluid by ion-pair RP-HPLC
del Moral, P.G. / Arin, M.J. / Resines, J.A. / Diez, M.T.
Journal of Chromatography B, 855 (2) Aug 2007:276-279
Abstract:
An isocratic ion-pair high-performance liquid chromatography (IP-RP-HPLC) method with UV detection was developed to identify and quantify ellagic acid (EA). This phenolic compound is widely distributed in the plants and is often present in the diet of ruminants. The method was validated and validation parameters were: linearity range 5–100 mg/L; correlation coefficient, 0.9995; mean recoveries (99.94 and 101.07%) and detection limit 1.4 mg/L. Method was applied for the determination of ellagic acid in oak leaves and in ruminal fluid from to a vitro ruminal system. The proposed method proved to be rapid and accurate and can be successfully used in ruminant nutrition studies.
Extraction and determination of ellagic acid cintentin chestnut bark and fruit
Food Chemistry, 110 (4) Oct 2008:1007-1011
Abstract:
Chestnuts are an important economic resource in the chestnut growing regions, not only for the fruit, but also for the wood. The content of ellagic acid (EA), a naturally occurring inhibitor of carcinogenesis, was determined in chestnut fruits and bark. EA was extracted with methanol and free ellagic acid was determined by HPLC with UV detection, both in the crude extract and after hydrolysis. The concentration of EA was generally increased after hydrolysis due to the presence of ellagitannins in the crude extract. The concentration varied between 0.71 and 21.6 mg g−1 (d.w.) in un-hydrolyzed samples, and between 2.83 and 18.4 mg g−1 (d.w.) in hydrolyzed samples. In chestnut fruits, traces of EA were present in the seed, with higher concentrations in the pellicle and pericarp. However, all fruit tissues had lower concentrations of EA than had the bark. The concentration of EA in the hydrolyzed samples showed a non-linear correlation with the concentration in the unhydrolyzed extracts.
Effects of Season, Variety, and Processing Method on Ellagic Acid Content in Pomegranate Leaves
Xiang, L. / Xing, D. / Lei, F. / Wang, W. / Xu, L. / Nie, L. / Du, L.
Tsinghua Science & Technology, 13 (4) Aug 2008:460-465
Abstract:
Ellagic acid (EA) has aroused great interest worldwide owing to its antioxidant, anti-inflammatory, and anticarcinogenetic properties. The EA content in pomegranate leaf was measured in this study using high performance liquid chromatography to investigate the effects of season, variety, and processing method on the EA level. The results show that the EA content in 11 varieties of pomegranate from the Zaozhuang region in
Key words: pomegranate leaf; ellagic acid; effect of season; variety; processing method; high performance liquid chromatography (HPLC)
12.
ELLAGIC ACID IN CHESTNUT WOOD
S.A. Vekiari, H. Lambrinea, P. Merkouri, M. Gordon, P. García-Macías
Abstract:
Ellagic acid (EA) is a naturally occurring polyphenolic acid which is found in 46 different fruits and other plant foodstuffs. Most bark, including that of chestnuts, is rich in polyphenolic compounds which may function to protect the trees from pathogens and pests. In the present study, chestnut wood from five Greek areas was extracted, and the amount of ellagic acid (EA) present before and after hydrolysis was analyzed using an HPLC procedure and UV detection. We found that the amount of EA was generally greater after hydrolysis. There were also great differences in the EA content of bark samples come from different areas of
Quantitification of gallic acid and ellagic acid from longan (Dimocarpus longan Lour.) seed and mango (Mangifera indica…
Soong, Y.Y. / Barlow, P.J.
Food Chemistry, 97 (3)Aug 2006:524-530
Abstract:
Gallic acid (GA) and ellagic acid (EA) have been identified in longan seed and mango kernel by the use of reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with photodiode array detection (DAD). The ethanolic extract of longan seed contained 23.3 and 156 mg/100 seeds of GA and EA, respectively. The ethanolic extracts of mango kernel contained approximately 87% more GA than the longan seed ethanolic extracts but about 32% less EA. After heat treatment and acid hydrolysis, mango kernel had higher concentrations of GA and EA, contributing to more potent antioxidant activity. The results demonstrated rich sources of GA and EA in longan seed and mango kernel which might provide a novel source of these natural antioxidants.
Transport behavior of ellagic acid of pomegranate leaf tannins and its correlation with total cholesterol alteration in Hep G2 cells.
Lan, Jiaqi / Lei, Fan / Hua, Lei / Wang, Yugang / Xing, Dongming / Du, Lijun
Biomedical chromatography : BMC, 23 (5) May 2009:531-536
Abstract:
The aim of this study was to investigate whether ellagic acid in pomegranate leaf tannins could be transported into HepG2 cells and its transport behavior. High-performance liquid chromatography coupled with a 996 photodiode array detector at 254 nm was applied. The mobile phase was an acetonitrile-water solution (containing 0.1% triethylamine, pH 3.0; 16:64, v/v, for determining ellagic acid in cells). The flow rate was 0.8 mL/min. Cells were incubated with pomegranate leaf tannins with 100 and 50 microg/mL (containing 1.71 and 0.85 microg/mL of ellagic acid, respectively) for a specific time, then lysed and sonicated in methanol to extract intracellular ellagic acid. A 10 microL aliquot of sample was injected into the HPLC system to determine ellagic acid concentration. The results showed that ellagic acid in pomegranate leaf tannins could be transported into the cells, which was in correlation with total cholesterol alteration in the cells. This is the first time that the transport behavior of ellagic acid through HepG2 cells in vitro has been comprehensively demonstrated.
Isolation, purification and identification of ellagic acid derivatives, catechins, and procyanidins fron the root bark…
Khallouki, F. / Haubner, R. /
Food and Chemical Toxicology, 45 (3) Mar 2007:472-485
Abstract:
The root bark of Anisophyllea dichostyla R. Br. is traditionally used in the Democratic Republic Congo for the treatment of several conditions such as anorexia, fatigue and intestinal infections. We have identified and quantitated several polyphenol antioxidants in the methanol extract of the root bark (120 g). The polyphenol content (3.32 g/kg) was predominantly ellagitannins (25%) and polyhydroxyflavan-3-ols (catechins and procyanidins, 75%) with 3′-O-methyl-3,4-methylenedioxo ellagic acid 4′-O-β-d-glucopyranoside and (–)-epicatechin as the major species in each class. These two compounds and the following species were identified unequivocally by NMR spectroscopy: (+)-catechin, (−)-epicatechin 3-O-gallate, 3-O-methyl ellagic acid, 3,3′-di-O-methyl ellagic acid, 3′-O-methyl-3,4-methylenedioxo ellagic acid, 3′-O-methyl-3,4-methylenedioxo ellagic acid 4′-O-β-d-glucopyranoside, and 3′-O-methyl ellagic acid 4-O-β-d-xylopyranoside. The following additional compounds were purified by semi-preparative HPLC and tentatively identified on the basis of UV spectra, HPLC–ESI-MS and nano-ESI-MS–MS: (+)-catechin-3-O-β-d-glucopyranoside, epicatechin-(4β → 8)-catechin (procyanidin B1), epicatechin-(4β → 8)-epicatechin (procyanidin B2), an (epi)catechin trimer, 3-O-methyl ellagic acid 4-O-β-d-glucopyranoside, (–)-epicatechin 3-O-vanillate, 3,4-methylenedioxo ellagic acid 4′-O- β-d-glucopyranoside, and 3,3′-di-O-methyl ellagic acid 4-O-β-d-xylopyranoside. Fractionation of the raw extract by column chromatography on silicic acid yielded 10 fractions. In the hypoxanthine/xanthine oxidase antioxidant assay system, CC-9 which contained a range of polyphenols dominated by (−)-epicatechin-O-gallate proved to be the most potent antioxidant fraction (IC50 = 52 μg/mL) in terms of ROS scavenging. In terms of XO inhibition CC-8, dominated by (epi)catechin trimer and which also contained appreciable amounts of 3′-O-methyl ellagic acid 4′-O-β-d-xylopyranoside, as well as the catechins (+)-catechin-3-O-β-d-glucopyranoside, epicatechin-(4β → 8)-catechin (procyanidin B1), and (−)-epicatechin 3-O-gallate, proved to be the most potent (IC50 = 36 μg/mL).
Keywords: Anisophyllea dichostyla; Antioxidant capacity; Chemoprevention; Ellagitannins; HPLC; HPLC–ESI-MS; NMR; Procyanidins; Xanthine oxidase
Fruit Juices: Ellagic Acid Concentration and Sensory Appeal
Kamau, Caroline M.
Pages : 89p.
Abstract
Dietary polyphenols such as ellagic acid (EA) are associated with decreased oxidative-stress related diseases. This study determined the concentration of EA in selected fruit juices and their blends. A sensory evaluation was conducted to determine the preferences, and overall acceptance of the juice blends by the panelists, based on color, mouthfeel, and sweetness. The juices were hydrolyzed and analyzed for EA concentration using HPLC-UV. Pomegranate juice had the highest concentration of EA (103 mg/L), while other juices ranged from 1 mg/L to 2 mg/L. Pomegranate-cranberry mixture had the highest EA concentration at 97 mg/L. Purple grape juice was in all the blends that ranked high in preferences and rated highest in the overall acceptance. Pomegranate juice was in all blends that ranked low in preferences and also lowest in overall acceptance. The results suggest that juices ranking high in color, sweetness and mouthfeel did not have high concentrations of EA.
Keywords : Apple juice; Cranberry juice; Ellagic acid; Ellagitannins; Overall acceptance; Pomegranate juice; Preference; Purple grape
Analytical methods for assay of ellagic acid its solubility studies
Bala,
Journal of Pharmaceutical and Biomedical Analysis, 40 (1) Jan 2006:206-210
Abstract:
Ultra-violet (UV) spectrophotometric and high performance liquid chromatographic methods for quantitative determination of ellagic acid (EA), an antioxidant were developed. The analytical methods were validated for linearity, accuracy, intra- and inter-day variability, and precision. EA was eluted using a polyethylene glycol (PEG) column with mobile phase composed of acetonitrile and 5 mM potassium dihydrogen orthophosphate buffer pH 2.5 (80:20, v/v). The UV and high performance liquid chromatography (HPLC) methods have lower detection limits of 0.2 and 0.1 μg/ml, respectively. Because of the increasing pharmaceutical interest in phytochemicals and their solubility problems, solubility studies for EA were also carried out. Various organic solvents and surfactants were screened for assessing solubility of EA and the compound was found to be soluble in some pharmaceutically acceptable solvents like triethanolamine, polyethylene glycol 400 and N-methyl pyrrollidone (NMP). Aqueous and pH dependent solubility of EA were determined using UV and HPLC methods, respectively.
Keywords: Antioxidants; Ellagic acid; Analytical method; HPLC; Validation; Solubility
18.
Isolation and characterization of ellagic acid derivatives isolated from Casearia sylvestris SW aqueous extract with…
Da Silva, S.L. / Calgarotto, A.K. / Chaar, J.S. / Marangoni, S.
Toxicon, 52 (6) Nov 2008:655-666
Abstrak:
The Casearia sylvestris SW (Flacourtiaceae) is utilized in folk medicine (
Analysis of ellagic acid in pomegranate rinds by capillary electrophoresis and high-performance liguid chromatography.
Zhou, Benhong / Wu, Zhenhua / Li, Xiaojun / Zhang, Jie / Hu, Xianming
Phytochemical analysis : PCA, 19 (1) Jan 2008:86-89
Abstract:
Two novel methods for the analysis of ellagic acid in pomegranate (Punica granatum) rinds are proposed. Capillary electrophoresis (CE) was performed in a bare fused-silica capillary using a buffer solution of tri(hydroxymethyl)aminomethane:potassium dihydrogen phosphate (pH 8.4) with an applied voltage of 20 kV and UV detection at 254 nm. HPLC analysis was performed with a Zobax SB C(18) column and a mobile phase consisting of methanol:ethyl acetate:potassium dihydrogen phosphate: phosphoric acid at a flow rate of 1.0 mL/min. Under optimised conditions, the HPLC retention and the CE migration times for ellagic acid were 10.32 and 12.23 min, respectively. Calibration curves of peak area vs. concentration gave correlation coefficients of 0.9999 for HPLC and 0.9990 for CE. The detection limits for HPLC and CE were 2.8 and 2.2 microg/mL, respectively. Average recoveries were 98.32 +/- 1.2% for HPLC and 96.52 +/- 2.8% for CE. Both methods were shown to be suitable for the determination of ellagic acid in pomegranate rinds extraction; however, the CE method required less solvent and gave better column efficiency, whilst the HPLC provided superior precision.
Isolation, purification and identification of ellagic acid derivatives, catechins, and procyanidins from the root bark of Anisophyllea dichostyla R. Br.
Khallouki, F / Haubner, R /
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 45 (3) Mar 2007:472-485
Abstract:
The root bark of Anisophyllea dichostyla R. Br. is traditionally used in the Democratic Republic Congo for the treatment of several conditions such as anorexia, fatigue and intestinal infections. We have identified and quantitated several polyphenol antioxidants in the methanol extract of the root bark (120g). The polyphenol content (3.32g/kg) was predominantly ellagitannins (25%) and polyhydroxyflavan-3-ols (catechins and procyanidins, 75%) with 3'-O-methyl-3,4-methylenedioxo ellagic acid 4'-O-beta-d-glucopyranoside and (-)-epicatechin as the major species in each class. These two compounds and the following species were identified unequivocally by NMR spectroscopy: (+)-catechin, (-)-epicatechin 3-O-gallate, 3-O-methyl ellagic acid, 3,3'-di-O-methyl ellagic acid, 3'-O-methyl-3,4-methylenedioxo ellagic acid, 3'-O-methyl-3,4-methylenedioxo ellagic acid 4'-O-beta-d-glucopyranoside, and 3'-O-methyl ellagic acid 4-O-beta-d-xylopyranoside. The following additional compounds were purified by semi-preparative HPLC and tentatively identified on the basis of UV spectra, HPLC-ESI-MS and nano-ESI-MS-MS: (+)-catechin-3-O-beta-d-glucopyranoside, epicatechin-(4beta-->8)-catechin (procyanidin B(1)), epicatechin-(4beta-->8)-epicatechin (procyanidin B(2)), an (epi)catechin trimer, 3-O-methyl ellagic acid 4-O-beta-d-glucopyranoside, (-)-epicatechin 3-O-vanillate, 3,4-methylenedioxo ellagic acid 4'-O- beta-d-glucopyranoside, and 3,3'-di-O-methyl ellagic acid 4-O-beta-d-xylopyranoside. Fractionation of the raw extract by column chromatography on silicic acid yielded 10 fractions. In the hypoxanthine/xanthine oxidase antioxidant assay system, CC-9 which contained a range of polyphenols dominated by (-)-epicatechin-O-gallate proved to be the most potent antioxidant fraction (IC(50)=52 micro g/mL) in terms of ROS scavenging. In terms of XO inhibition CC-8, dominated by (epi)catechin trimer and which also contained appreciable amounts of 3'-O-methyl ellagic acid 4'-O-beta-d-xylopyranoside, as well as the catechins (+)-catechin-3-O-beta-d-glucopyranoside, epicatechin-(4beta-->8)-catechin (procyanidin B(1)), and (-)-epicatechin 3-O-gallate, proved to be the most potent (IC(50)=36 micro g/mL).
High-performance liquid chromatographic determination with photodiode array detection of ellagic acid in fresh and processed fruits.
Amakura, Y / Okada, M / Tsuji, S / Tonogai, Y
Journal of chromatography. A, 896 (1-2) Oct 2000: 87-93
Abstract:
A high-performance liquid chromatographic (HPLC) procedure based on an isocratic elution with photodiode array detection has been developed for a simple and rapid determination of ellagic acid (EA) in fresh and processed fruits. The homogenized sample was refluxed with methanol and then the extract was refined using a solid-phase cartridge before HPLC. We analyzed EA in 40 kinds of fresh fruits and 11 kinds of processed fruits by the developed method. EA was found in several berries, fueijoa, pineapple and pomegranate. This is the first occurrence of the detection of EA in bayberry, fueijoa and pineapple.
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