Senin, 22 April 2013

Production of Membrane Proteins: Strategies for Expression and Isolation

Anne Skaja Robinson (editor)
Buku ini diterbitkan oleh Wiley-VCH tahun 2011. Cakupan 443 halaman.
Daftar isi:

Part I.  Expression Systems, 11

  • Bacterial Systems, 13
  •  Membrane Protein Expression in Saccharomyces cerevisiae, 37
  •  Expression Systems: Pichia pastoris, 75
  •  Heterologous Production of Active Mammalian G-Protein-Coupled Recep-tors Using Baculovirus-Infected Insect Cells, 109
  •  Membrane   Protein  Expression  in Mammalian Cells, 139
  •  Membrane Protein Production Using Photosynthetic Bacteria: A Practical Guide, 167

Part II. Two Protein-Specific Consi-derations, 199

  • Peripheral Membrane Protein Produc-tion for Structural and Functional Studies, 201
  •  Expression of G-Protein-Coupled Receptors, 219
  •  Structural Biology of Membrane Proteins, 249

Part III. Emerging Methods and Approaches, 275

  • Engineering Integral Membrane Proteins for Expression and Stability, 277
  •  Expression and Purification of G-Protein-Coupled Receptors for Nuclear Magnetic Resonance Structural Studies, 297
  •  Solubilization, Purification, and Characterization of Integral Membrane Proteins, 317
  •  Stabilizing Membrane Proteins in Detergent and Lipid Systems, 361
  •  Rapid Optimization of Membrane Protein Production Using Green Fluorescent Protein-Fusions and Lemo21(DE3), 391

Index, 407

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