Production of Membrane Proteins: Strategies for Expression and Isolation

Anne Skaja Robinson (editor)

Buku ini diterbitkan oleh Wiley-VCH tahun 2011. Cakupan 443 halaman.

Daftar isi:

Part I.  Expression Systems, 11

• Bacterial Systems, 13
•  Membrane Protein Expression in Saccharomyces cerevisiae, 37
•  Expression Systems: Pichia pastoris, 75
•  Heterologous Production of Active Mammalian G-Protein-Coupled Recep-tors Using Baculovirus-Infected Insect Cells, 109
•  Membrane   Protein  Expression  in Mammalian Cells, 139
•  Membrane Protein Production Using Photosynthetic Bacteria: A Practical Guide, 167

Part II. Two Protein-Specific Consi-derations, 199

• Peripheral Membrane Protein Produc-tion for Structural and Functional Studies, 201
•  Expression of G-Protein-Coupled Receptors, 219
•  Structural Biology of Membrane Proteins, 249

Part III. Emerging Methods and Approaches, 275

• Engineering Integral Membrane Proteins for Expression and Stability, 277
•  Expression and Purification of G-Protein-Coupled Receptors for Nuclear Magnetic Resonance Structural Studies, 297
•  Solubilization, Purification, and Characterization of Integral Membrane Proteins, 317
•  Stabilizing Membrane Proteins in Detergent and Lipid Systems, 361
•  Rapid Optimization of Membrane Protein Production Using Green Fluorescent Protein-Fusions and Lemo21(DE3), 391

Index, 407

 

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